What is the optimum temperature for the Geobacillus stearothermophilus
Hypothesis: The optimum temperature for the amylase obtained from Geobacillus stearothermophilus
is high, most probably above 60 ? since the bacteria mainly lives in high temperature environments such as hot springs.
If the temperature of the reaction environment rises above 60 ?, then the amount of maltose formed from the reaction of the bacterial amylase, will be the highest concentration.
Distilled water, Buffer,
5% starch solution, bacterial amylase, 3,5-Dinitrosalicylic acid (DNS)
12 test tubes, test tube holder, water bath, spectrophotometer, cuvettes, micropipettes.
For the determination of the effect of temperature on the activity of Geobacillus stearothermophilus
amylase, 6 test tubes are labelled and placed in a test tube rack.
450 ?l of distilled water is added to each test tube followed by the same amount of buffer solution that he...